TY  - JOUR
A1  - Montesinos, Marı́a  Luz
A1  - Foucher, Isabelle
A1  - Conradt, Marcus
A1  - Mainguy, Gaëll
A1  - Robel, Laurence
A1  - Prochiantz, Alain
A1  - Volovitch, Michel
T1  - The Neuronal Microtubule-Associated Protein 1BIs under Homeoprotein Transcriptional Control
Y1  - 2001/05/15 
JF  - The Journal of Neuroscience 
JO  - The Journal of Neuroscience 
SP  - 3350 
EP  - 3359 
DO  - 10.1523/JNEUROSCI.21-10-03350.2001 
VL  - 21 
IS  - 10 
UR  - http://www.jneurosci.org/content/21/10/3350.abstract 
N2  - To identify genes regulated by homeoprotein transcription factors in postnatal neurons, the DNA-binding domain (homeodomain) of Engrailed homeoprotein was internalized into rat cerebellum neurons. The internalized homeodomain (EnHD) acts as a competitive inhibitor of Engrailed and of several homeoproteins (Mainguy et al., 2000). Analysis by differential display revealed thatmicrotubule-associated protein 1B (MAP1B) mRNA is upregulated by EnHD. This upregulation does not require protein synthesis, suggesting a direct effect of the homeodomain onMAP1B transcription. The promoter region of MAP1Bwas cut into several subdomains, and each subdomain was tested for its ability to bind Engrailed and EnHD and to associate with Engrailed-containing cerebellum nuclear extracts. In addition, the activity, and regulation by Engrailed, of each subdomain and of the entire promoter were evaluated in vivo by electroporation in the chick embryo neural tube. These experiments demonstrate that MAP1B promoter is regulated by Engrailedin vivo. Moreover, they show that one promoter domain that contains all ATTA homeoprotein cognate binding sites common to the rat and human genes is an essential element of this regulation. It is thus proposed that MAP1B, a cytoskeleton protein involved in neuronal growth and regeneration, is under homeoprotein transcriptional regulation. 
ER  -