Statistical representation of astrocyte loss in TK transgenic mice. All counts were performed on 50 μm vibratome midline sagittal sections and included only the distal 500 μm of the folia. Shown are box plots of mean and variance of astrocyte number (a) or density (b) in the culmen and central lobule. Squares incenter of boxes represent the mean in that group of data. The second and third quartile are represented by the area above and below thecenter line, respectively. The barsextend to the minimum and maximum. a, With mean cell counts in the control folia of 601 and 625 for the central lobule and culmen, respectively, nested ANOVA suggested an average cell loss of 354 ± 54 in the central lobule and 360 ± 48 in the culmen (99.5% confidence interval). b, With mean density in the control of 2202 and 2081 astrocytes/mm² for the central lobule and culmen, respectively, nested ANOVA suggested an average decrease in density of 876 ± 393 in the central lobule and 386 ± 116 in the culmen (99.7% confidence interval).