A, Nucleotide sequence of amplified monkey dorsal raphe TPH cDNA compared to rat (Kim et al., 1991) and human TPH (Boularand et al., 1990). The coding sequence is numbered from nucleotide 1. Underlined regions indicate the location of PCR primers. Bold letters indicate differences in the nucleotides between sequences. Nucleotides that are identical to those in monkey TPH appear as dashes.B, Plasmid map of monkey 5′ TPH cDNA showing restriction enzyme recognition and promoter sites. pCR II was the 3.9 kb plasmid used for ligation of the 0.25 kb monkey TPH cDNA. The cDNA clone was oriented for antisense transcription by SP6 RNA polymerase and linerized for transcription via a BbsI digest. Restriction endonuclease BamHI was used to linearize the plasmid for coding sequence. T7 RNA polymerase was then used to make sense cDNA.