A, Average number of above-threshold pixels measured in spayed and steroid-treated animals (n = 5 animals/treatment group) with five levels per animal. B, The fraction of pixels that are the result of specific labeling in the dorsal raphe over total pixel number in spayed, E-treated, and E+P-treated animals.Asterisks, Significantly different from spayed;p < 0.05. There were no significant differences between E- and E+P-treated groups.