Glutamate immunoreactivity: electron microscopic immunocytochemical analysis
| Structure | Immunogold distribution | Dot distribution (random) | χ2 | ||
|---|---|---|---|---|---|
| n | n | % | |||
| Cellular distribution | |||||
| Varicosities | 182 | 42 | 175 | 16 | 185.063-150 |
| Perikarya | 100 | 23 | 229 | 20 | 1.11 |
| Neurites | 108 | 25 | 475 | 43 | 32.71 |
| Gila | 48 | 10 | 236 | 21 | 21.77 |
| Totals | 438 | 100 | 1115 | 100 | Σ = 240.63-150 |
| Subcellular distribution in varicosities | |||||
| Synaptic vesicles | 182 | 65 | 57 | 26 | 162.753-150 |
| Large, dense- core vesicles | 13 | 5 | 21 | 9 | 7.26 |
| Mitochondria | 25 | 9 | 23 | 11 | 0.83 |
| Cytosol | 55 | 19 | 91 | 42 | 32.0 |
| Filaments | 5 | 2 | 6 | 3 | 0.57 |
| Plasma | 0 | 0 | 13 | 6 | 17.0 |
| Membrane | |||||
| Vacuoles | 0 | 0 | 8 | 4 | 10.0 |
| Totals | 280 | 100 | 281 | 100 | Σ = 230.413-150 |
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The structures underlying immunogold particles were identified, and the number of particles associated with each structure was counted. The distribution of immunogold particles was then compared, by means of a χ2 test, with that of a grid of dots superimposed over the same micrographs. The distribution of the dots was taken as that expected for a random distribution of label. The distribution of immunogold particles over cellular structures was not random (p < 0.001). Because varicosities were labeled, the distribution of immunogold particles over subcellular structures within varicosities was compared with that of a random distribution of dots. The distribution of immunogold particles over subcellular structures within the varicosities was not random (p < 0.001).
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↵F3-150 Significantly more than expected; p < 0.001.
