Oxidative insults and HNE induce rapid and delayed cell death in PC12 cells: Bcl-2 prevents delayed cell death.A, B, PC12-V (A) and PC12-Bcl2 (B) cells were exposed to the indicated concentrations of HNE, Aβ, or FeSO₄ for the indicated time periods, and levels of MTT reduction were quantified (mean and SEM of determinations made in four culture wells/condition).C, D, PC12-V cells (C) and PC12-Bcl2 cells (D) were exposed to vehicle (Control) or the indicated concentrations of HNE, Aβ, orFeSO₄, and trypan blue-positive and nonstaining cells were counted. Values are the mean percentage of trypan blue-positive cells in four separate cultures.