Oxidative insults induce membrane lipid peroxidation in neural cells: suppression by Bcl-2. A, Levels of TBARS fluorescence were quantified in PC12-V cells, PC12-Bcl2 cells, and hippocampal neurons after 4 hr of exposure to vehicle (Control), FeSO4 (1 mm PC12 cells, 2 μm hippocampal neurons),Aβ (50 μm PC12 cells, 10 μm hippocampal neurons), or HNE (10 μm PC12 cells, 2 μm hippocampal neurons). Values represent the mean and SEM of determinations made in four to six separate cultures. B, Hippocampal cell cultures were exposed to 1 μm HNE or 2 μmFeSO4, and HNE levels in the culture medium and cells were quantified at the indicated time points. Values represent the mean and SEM of determinations made in four separate cultures.
