GSH protects PC12 cells against apoptosis induced by oxidative insults and HNE. A, Cultures of PC12-V cells were exposed for 70 hr to Vehicle, 10 μmHNE, 50 μmAβ, or 1 mmFeSO4 in the absence (Control) or presence of 1 mmGSH. Then cultures were fixed and stained with Hoescht dye, and the numbers of cells with condensed and fragmented nuclei were counted. Values are the mean and SEM of determinations made in four separate cultures. *p < 0.01 compared with vehicle control value; **p < 0.01 compared with corresponding control value (ANOVA with Scheffé’s post hoc tests). B, Cultures were exposed for 6 hr to vehicle (Cont), 50 μmAβ, 1 mmFeSO4, 10 μmHNE, or 300 μm buthionine sulfoximine (BSO). Then relative levels of GSH were determined by using the monochlorobimane fluorescence method. Values are the mean and SEM of determinations made in four separate cultures. *p < 0.01 compared with corresponding value for PC12-V cells (ANOVA with Scheffé’s post hoctests).
