Temporal and Spatial Expression Patterns of Transgenes Containing Increasing Amounts of the Drosophila Clock Geneperiod and a lacZ Reporter: Mapping Elements of the PER Protein Involved in Circadian Cycling

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Fig. 4.
Fig. 4.

per-Expressing cells in the head of a per+ XLG-B transgenic adult at ZT21. The sections of the male were stained with anti-β-GAL antibody (staining of this line is more intense than in XLG-A, but the spatial pattern is identical; compare Fig. 10) (also data not shown).A, Six LNd cells (open arrow) are visible in this section (diameter, ∼5–6 μm); the solid arrow points to two large cells (diameter, ∼11 μm) from the relatively dorsal region of the LNv group;arrowheads indicate glia cells bordering the medulla. Scale bar, 12.5 μm. B, Higher magnification ofA. Scale bar, 8 μm. C, The solid arrow points to LNv; four cells of smaller size (diameter, ∼4–5 μm) are visible in this plane. D, DNs (arrow); note that the nucleus seems stained lighter than the surrounding cytoplasm (diameter, ∼3–5 μm). Magnifications in C and D are as in A. E, PR staining, which appears mainly cytoplasmic (the nuclei cannot be distinguished from the soma); the open arrow points to a group of LNd. Scale bar, 25 μm.

  1. The Journal of Neuroscience vol. 17 no. 2 676-696
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