Caveolin-related proteins are detected in detergent-insoluble complexes isolated from adult cerebellum and cortex. A, B, Detergent-insoluble complexes were isolated from adult cerebellum (A) and adult cortex (B) as described in Materials and Methods. Equal volumes of fractions collected across the gradients were separated by one-dimensional SDS-PAGE and processed for immunoblot analyses using the anti-caveolin polyclonal antibody. A single band of appropriate molecular mass for caveolin was detected for both cerebellum (A) and cerebral cortex (B) in a peak that corresponded to the position of the opaque band observed after centrifugation, ∼20% sucrose.C, D, Samples from the peak caveolin fraction (fraction 7) were resolved by two-dimensional SDS-PAGE and processed for immunoblot analyses using the anti-caveolin polyclonal antibody. The immunoreactive polypeptide profile observed for cerebellum (C) and cortex (D) was identical and comprised two major proteins between 22 and 31 kDa with isoelectric points between pH 5 and 6. Molecular mass × 10−3 is indicatedvertically; fraction numbers are indicatedhorizontally in A and B; pH values are indicated horizontally in Cand D.
