V-ANedd protects sympathetic neurons from NGF withdrawal, but not from oxidative stress. A, V-ANedd protects sympathetic neurons from NGF withdrawal. At the time of NGF deprivation, V-ANedd (400 nm) was added to the cultures, as indicated. Numbers of surviving neurons were determined at the indicated times, as described in Materials and Methods, and are reported as relative to the number present in each culture at the time of NGF withdrawal. B, V-ANedd does not protect sympathetic neurons from death induced by SOD1 downregulation and nitric oxide generation. Sympathetic neurons, after 6 d in culture, were maintained with NGF (100 ng/ml) and mixtures of the following additives as indicated: V-ASOD1 (50 nm), SNAP (100 μm), andV-ANedd (400 nm). Numbers of surviving neurons were determined at the indicated times, as above.