Morphology of cells rescued by V-ANedd. Photomicrographs of cells treated as described in the preceding figures. Top row, Naive PC12 cells:left, in serum-free RPMI 1640 medium with 100 ng/ml NGF (24 hr); middle, in RPMI 1640 medium alone (24 hr);right, in serum-free RPMI 1640 medium with 400 nm V-ANedd (24 hr). Middle row,Neuronal PC12 cells: left, replated in serum-free RPMI 1640 medium with 100 ng/ml NGF (24 hr);middle, replated in serum-free RPMI 1640 medium without NGF (24 hr); right, replated in serum-free RPMI 1640 medium with 400 nm V-ANedd (24 hr). Bottom row, SCG (sympathetic) neurons:left, cultured with 100 ng/ml NGF (3 d);middle, cultured in NGF-free medium with anti-NGF (3 d);right, cultured in NGF-free medium with anti-NGF plus 400 nm V-ANedd (3 d). Phase contrast optics, 80× magnification.