Colocalization of mAChR with markers for retinal neurons and Müller glia. Embryonic retinal cultures were prepared from E9 retina and grown on poly-d-lysine-coated glass slides for 5 d. Cells were prepared for immunocytochemistry, as described in Materials and Methods. mAChR were immunolabeled with FITC-conjugated secondary antibody (shown on the leftside of each pair), and retinal cell markers were immunolabeled with rhodamine-conjugated secondary antibody (shown on theright side of each pair). See Materials and Methods and Table 1 for specificity of marker antigens. All photographs were taken at 400× magnification. Colocalizations shown include the following:A, cm2 and neurofilament colocalizing in ganglion cells;B, cm2 and IRBP colocalizing in photoreceptors;C, cm2 and Islet-1 colocalizing in ganglion cells;D, cm3 and neurofilament colocalizing in ganglion cells;E, cm3 and protein kinase C (α and β) colocalizing in bipolar cells; F, cm3 and Islet-1 colocalizing in ganglion cells; G, cm4 and neurofilament colocalizing in ganglion cells; H, cm4 and vimentin colocalizing in Müller glia; I, cm4 and tenascin colocalizing in amacrine and horizontal cells.