Neuronal specificity of rAAV–MD–TH Expression
| rAAV vector | Neuronal specificity | Astrocytic specificity | ||||
|---|---|---|---|---|---|---|
| n | TH+ cells sampled | NeuN+/TH+ doubled stained (%) | n | TH+ cells sampled | GFAP+/TH+ doubled stained (%) | |
| MD–TH | 3 | 414 | 92.5 | 4 | 786 | 1.3 |
| MD–TH + MD–GTPCHI | 3 | 1187 | 96.2 | 3 | 807 | 1.2 |
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At least 100 TH+ cells were sampled per animal.n refers to the number of animals from which TH+cells were sampled for each vector group. On the left half of the table, TH+ cells sampled indicates the total number of TH+ cells in each group that were scored for NeuN immunoreactivity. The larger number of cells sampled for the MD–TH + MD–GTPCHI group is coincidental and does not signify that there were more TH+ cells in those animals than in the MD–TH group. The percentage of TH+ cells that were also NeuN+ for each group sampled is shown in the fourth column. On the right half of the table, TH+ cells sampled indicates the total number of TH+ cells in each group that were scored for GFAP immunoreactivity. The percentage of TH+cells that were also GFAP+ for each group sampled is shown in the last column.
