Mapping ofVoila1. A, In situ chromosomal mapping of the PGAL4 insertion in salivary gland of w;Voila1/+ larva. B, Deficiencies used to map the bisexual courtship observed inVoila1/TM3 males. Deficiencies are aligned with the salivary gland chromosome map (Lindsley and Zimm, 1992). Bars represent the extent of the deficiencies, and the hatched region represents uncertainty as to breakpoint position. The double-arrowed line indicates the chromosomal region 86D4–E19. C, Deficiency mapping of the behavioral phenotype ofVoila1/TM3. Courtship responses of w; Df(3R)/TM3 males toward decapitated male or female targets were measured. The height of each bar indicates the mean (±SE) courtship index measured in at least 25 individuals per genotype (15 for w; +/TM3). All deficiencies were tested over the TM3, Sb Ser balancer chromosome from theVoila1/TM3 strain. Sexual discrimination (difference between the intensity of homo- and heterosexual courtship) was statistically tested (two-factor ANOVA, Newman–Keuls test) for each genotype. ***p < 0.001. ns, Not significant.
