In vivo effect of PKC inhibitor Gö 7874 on the transient, stimulus-induced PKC activation
| Stimulation | PBS | Gö 7874 |
|---|---|---|
| − | 1.00 ± 0.05 (20) | 1.12 ± 0.06 (21) |
| CS | 1.36 ± 0.05 (32)* | 0.95 ± 0.04 (21) |
| US | 1.32 ± 0.06 (16)* | 1.05 ± 0.07 (12) |
| 1× (CS-US) | 1.43 ± 0.06 (21)* | 1.06 ± 0.08 (7) |
| 1× (US-CS) | 1.36 ± 0.05 (38)* | 0.96 ± 0.06 (6) |
| 3× (CS-US) | 1.47 ± 0.05 (21)* | 1.00 ± 0.08 (8) |
| 3× (US-CS) | 1.42 ± 0.06 (15)* | n.d. |
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Animals were injected with 1 μl Gö 7874 (1 mm) or PBS and received different stimulations 20 min later [(CS-US), forward pairing; (US-CS), backward pairing]. AL were prepared immediately after stimulation, and PKC activity was determined in the presence of calcium and DAG. In each experiment, values were normalized to PKC activity measured in PBS-injected, unstimulated animals. Mean values are presented as SEM. The number of measurements is shown in parentheses. Values significantly different from the corresponding PKC activity in unstimulated animals are marked by an asterisk (p < 0.05; t test).
