Table 1.
Effect of 5,7-DHT or pCPA treatment on tissue 5-HT, DA, and NA concentrations from various rat brain regions
| 5,7-DHT | pCPA | |||
|---|---|---|---|---|
| Sham-operated | Treated | Saline | Treated | |
| Dorsal hippocampus | ||||
| 5-HT | 3.51 ± 0.25 | 0.21 ± 0.13*** | 3.18 ± 0.28 | 0.20 ± 0.07*** |
| NA | 4.33 ± 0.74 | 3.11 ± 0.46 | 3.44 ± 0.23 | 1.46 ± 0.12*** |
| NAC | ||||
| 5-HT | 3.86 ± 0.39 | 0.19 ± 0.12*** | 3.69 ± 0.25 | 0.24 ± 0.03*** |
| DA | 88.39 ± 10.26 | 75.52 ± 5.19 | 80.71 ± 6.09 | 43.68 ± 5.04*** |
| NA | n.d. | n.d. | n.d. | n.d. |
| Striatum | ||||
| 5-HT | 2.92 ± 0.35 | 0.08 ± 0.06*** | 1.89 ± 0.11 | 0.09 ± 0.03*** |
| DA | 103.39 ± 7.17 | 92.33 ± 6.33 | 94.02 ± 8.34 | 76.56 ± 4.39 |
| NA | n.d. | n.d. | n.d. | n.d. |
| SN | ||||
| 5-HT | 12.20 ± 3.38 | 0.61 ± 0.06*** | 14.55 ± 1.67 | 0.87 ± 0.25*** |
| DA | 12.25 ± 1.70 | 12.28 ± 1.44 | 11.25 ± 2.73 | 14.06 ± 1.81 |
| NA | 5.03 ± 0.60 | 5.31 ± 0.36 | 4.65 ± 0.33 | 6.13 ± 0.64 |
| VTA | ||||
| 5-HT | 9.44 ± 1.08 | 0.27 ± 0.12*** | 6.10 ± 0.73 | 1.02 ± 0.12*** |
| DA | 8.72 ± 1.09 | 9.15 ± 2.30 | 12.54 ± 3.46 | 14.64 ± 2.28 |
| NA | 9.88 ± 1.07 | 8.34 ± 0.56 | 10.44 ± 1.39 | 9.7 ± 0.74 |
-
Each value, expressed in ng/mg proteins, represents the mean ± SEM of five or six independent determinations. Measurements were performed 21 d after intra-raphe 5,7-dihydroxytryptamine (5,7-DHT) or vehicle injection (sham-operated rats) and 24 hr after the last intraperitoneal administration of para-chlorophenylalanine (pCPA) or saline. ***p < 0.001 versus control animals (two-tailed Student’s t test). NAC, Nucleus accumbens; SN, substantia nigra; VTA, ventral tegmental area; n.d., not determined.
