Effect of 5,7-DHT or pCPA treatment on absolute basal levels of DA, DOPAC, and 5-HIAA in dialysates from the NAC and the striatum
| 5,7-DHT | pCPA | |||
|---|---|---|---|---|
| Sham-operated | Treated | Saline | Treated | |
| NAC | ||||
| DA | 6.9 ± 0.9 | 8.1 ± 1.4 | 6.2 ± 0.9 | 2.9 ± 0.3*** |
| DOPAC | 2956 ± 623 | 2843 ± 459 | 2609 ± 376 | 1665 ± 123* |
| 5-HIAA | 626 ± 43 | 29 ± 3.1*** | 665 ± 62 | 101 ± 16*** |
| Striatum | ||||
| DA | 15.6 ± 3.5 | 18.8 ± 3.4 | 14.3 ± 2.4 | 7.2 ± 0.9* |
| DOPAC | 3274 ± 716 | 4519 ± 590 | 2764 ± 491 | 1622 ± 174* |
| 5-HIAA | 957 ± 109 | 25 ± 3.5*** | 827 ± 68 | 114 ± 16*** |
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Each value, expressed in pg/30 μl dialysates, represents the mean ± SEM of five or six independent determinations of DA, DOPAC, and 5-HIAA basal levels in dialysates collected from the nucleus accumbens (NAC) and the striatum before dorsal raphe nucleus (DRN) electrical stimulation. Experiments were performed 18–21 d after intra-DRN 5,7-dihydroxytryptamine (5,7-DHT) or vehicle injection (sham-operated rats) and 24 hr after the last intraperitoneal administration of para-chlorophenylalanine (pCPA) or saline. *p < 0.05 and ***p < 0.001 versus control animals (two-tailed Student’s t test).
