Cer-induced I_Kir inhibition involves Ras-dependent pathways. A, Representative traces before (pre) and after (post) perfusion with 10 μm cer. Only currents at −80 mV are shown for simplicity in this and subsequent figures. IgG (+), Inclusion of 0.2 μg/ml control IgG in the intracellular (pipette) solution. RasAb (+), Inclusion of 0.2 μg/ml neutralizing antibody against Ras (Y13-259) in the pipette solution.B, Summarized data showing I_Kirmodulation by cer (10 μm) but not by inactive cer analog dh-cer (10 μm) (*p < 0.0001 for overall ANOVA; p < 0.0001 for ctrl vs cer; p< 0.001 for cer vs dh-cer). Y13-259 prevented the effect of cer onI_Kir (**p < 0.02 for Y13-259 vs ctrl IgG).