Cer-induced I_Kir inhibition involves raf-1-dependent pathways. A, Western blot analysis of raf-1 protein expression in cultures treated with 5 μmsense, antisense, or nonsense raf-1 ODNs. Raf-1 protein was detected using a monoclonal antibody against raf-1. Data shown are representative of three experiments. B, Summarized data showing that cer-induced I_Kir inhibition was prevented by antisense raf-1 ODN but not by sense or nonsense raf-1 ODNs (*p < 0.03 for antisense ODNs vs sense and nonsense ODNs). See Materials and Methods for details.