Table 1.
Kinetic and pharmacological properties of the basket cell GABAARs
| n | Rise time20%/80%(μsec) | τ1 (msec) | τ2(msec) | A1/(A1 + A2) (%) | |
|---|---|---|---|---|---|
| 1 mm GABA (1 msec pulse) | 11 | 412 ± 32 | 2.2 ± 0.1 | 80.3 ± 3.2 | 75.3 ± 6.7 |
| 1 mm GABA (100 msec pulse) | 52 | 559 ± 12 | 2.4 ± 0.1 | 61.8 ± 1.7 | 78.8 ± 6.3 |
| 3 mm GABA (100 msec pulse) | 7 | 474 ± 41 | 2.1 ± 0.1 | 53.5 ± 1.6 | 79.4 ± 6.2 |
| 300 μm GABA (100 msec pulse) | 2 | 1017 ± 301 | 2.5 ± 0.1 | 49.1 ± 4.2 | 70.0 ± 17.8 |
| 100 μm GABA (100 msec pulse) | 5 | 2714 ± 593 | 64.7 ± 11.4 | ||
| 30 μm GABA (100 msec pulse) | 5 | 12001 ± 6654 | 89.9 ± 24.3 | ||
| 1 mm GABA + 1 μm Flu (100 msec pulse) | 6 | 508 ± 21 | 2.4 ± 0.1 | 73.4 ± 2.7 | 78.9 ± 6.8 |
| 100 μmGABA + 1 μm Flu (100 msec pulse) | 4 | 1298 ± 45 | 4.4 ± 0.1 | 70.7 ± 6.4 | 57.2 ± 4.6 |
| EC50 (peak) | Hill coefficient (peak) | EC50 (slow component) | Hill coefficient (slow component) | ||
|---|---|---|---|---|---|
| GABA dose–response curve | 10 | 139 ± 29 μm* | 1.0 ± 0.2 | 24 ± 2 μm* | 2.0 ± 0.4 |
| + 1 μm flunitrazepam | 6 | 57 ± 6 μm* | 0.6 ± 0.0 | 5 ± 2 μm* | 1.8 ± 0.6 |
| + 100 μmZn2+ | 8 | 167 ± 23 μm | 0.8 ± 0.1 |
| IC50 (peak) | IC50 (slow component) | ||||
|---|---|---|---|---|---|
| GABA predesensitization | 8 | 642 ± 105 nm | 1.6 ± 0.4 | 629 ± 96 nm | 1.6 ± 0.3 |
| Zn2+block dose–response curve | 8 | 8 ± 3 μm | 0.5 ± 0.1 | 16 ± 7 μm | 0.8 ± 0.3 |
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*Significant differences (p < 0.01).
