Anterograde labeling of axons in the retino-retinal projection. A, Schematic showing that injection of 4DiI-10ASP into the left eye labels the axons entering the temporal contralateral retina. The intersections at the left optic fissure (OF) indicate the location ofB–D. B, Axons projecting from theOF toward the peripheral retina at E9. C, Large number of axons extending from the OF toward the temporal retinal periphery. D–G, Higher magnifications of axonal tips showing the typical growth cones within the retina contralateral to the dye injection. Scale bars: B, C, 50 μm; D–G, 25 μm.