Developmental Northern blots forDrosophila ankyrins. Total RNA (40 μg) was separated on a 1% formaldehyde agarose gel and blotted onto nylon membranes, which were probed with ³²P-labeled DNA probes specific for either Dank1 (A) or Dank2 (B). Lanes 1 contain RNA isolated from Drosophila S2 tissue culture cells, lanes 2 from 0–12 hr embryos, lanes 3 from 12–24 hr embryos, lanes 4 from larvae, lanes 5from pupae, and lanes 6 from adult flies.