Vti1a-β copurified with clathrin-coated vesicles isolated from nerve terminals. Clathrin-coated vesicles (CCVs) were isolated from synaptosomes with brain homogenate as the starting material. Highly enriched clathrin-coated vesicles were isolated from the high-speed pellet of lysed synaptosomes (Ficoll load) via the fractions Ficoll SN and D₂O load. Vti1a-β copurified in parallel with the synaptic vesicle markers synaptobrevin (Syb), synaptophysin (Syp), and SCAMP in clathrin-coated vesicles. Clathrin light chain (CLC) was highly enriched. Very low amounts of Vti1b were found in clathrin-coated vesicles.