Comparison of percent change of IPSC amplitude at 90 min in each experimental group. At V_HOLD ≤ −55 mV, LFS produced a significant change in IPSC amplitude as compared with no stimulation (No Stim; p < 0.05; t = symbol 45 2.16; df = 14) or LFS + BAPTA (p < 0.05; t = 2.1; df = 15). At V_HOLD = 0 mV, LFS at P7–P12 produced a significant change in IPSC amplitude as compared with no LFS (p < 0.0005; t = symbol 45 3.6; df = 29), no stimulation (p < 0.05; t = symbol 45 3.6; df = 29), LFS at P17–P19 (p < 0.0005; t= symbol 45 4.14; df = 28), or LFS + BAPTA (p < 0.0001; t = 3.6; df = 31). Finally, LFS induced a significantly greater reduction in IPSC amplitude when delivered at V_HOLD = 0 as compared with V_HOLD ≤ −55 (p < 0.05; t = 2.23; df = 29). The mean percent change was calculated by comparing the average IPSC amplitude recorded at 50–60 min after LFS with the initial IPSC amplitude.