EphA4 immunostaining in transverse (A, B), coronal (D–F), and sagittal (C) paraffin sections at stages 31 (E7, A), 34 (E8,B), 37 (E11, C, D), 45 (E20, E), and P7 (F). Parasagittal banded EphA4 expression is apparent at E8 (B) and persists to posthatch (F).Asterisk in A refers to the segment with the lower level of EphA4 expression. Roman numeralsrefer to cerebellar lobules. A–E in Band C refer to Purkinje cell parasagittal bands labeled alphabetically from the midline. cbn, Deep cerebellar nuclei; cc, ventral cerebellar commissure;eff, corticonuclear efferents; egl, external granular layer; L, lateral subdivision;M, medial subdivision; V, ventricle. Scale bar, 200 μm.