Double immunostaining of EphA4 (Band D) and calbindin (A and C) in coronal plane of frozen sections of a stage 40 (E14) cerebellum. A, B, Purkinje cell band, C, is devoid of EphA4 labeling but is calbindin positive. C, D, In most areas, EphA4 and calbindin bands colocalize. B,C, and D in A andB refer to Purkinje cell parasagittal bands. Scale bar, 100 μm.