Anti-Shaker is found in axonal compartments located in the nerves of the STNS. A, Diagram of the STNS. Lines represent nerves,filled ovals represent ganglia, andrectangles represent muscles. COG, Commissural ganglion; EOG, esophogeal ganglion;ion, inferior esophogeal nerve; son, superior esophogeal nerve; lpn, lateral pyloric nerve;pyn, pyloric constrictor nerve; IC, inferior cardiac. B, Electron micrograph of STNS axon with surrounding glial sheath; we gratefully acknowledge that the EM photograph was a kind gift from Dr. David King. gn, Glial cell nucleus. Line with double arrowheads shows the thickness of the glial wrap at one point tangential to the axon.C, Horizontal optical section (13.3-μm-thick) from the center of a representative anti-Shaker-stained ganglion–dvn showing the emergence of the dvn from the STG. Arrowsapproximate the preaxon region (between the end of the neuropil and the beginning of the dvn). Note that the lack of anti-Shaker staining in the neuropil processes continues as the processes leave the ganglion and enter the dvn, whereas the perineural sheath (PS) is brightly stained. D, Horizontal optical section from the distal region of an anti-Shaker-stained dvn. Note the continuous stain along the edges of large axons in the plane of focus and the fainter staining in the surrounding glial sheaths when they are in the plane of focus. The faintly stained bundle of small-diameter axons is seen just below center. The optical section is ∼6 to 8-μm-thick.E, High magnification of a single process and the surrounding glial sheath from an anti-Shaker-stained lvn.Arrows mark the outer bounds of the glial sheath. The thickness of the optical section is ∼0.5 μm.F, Anti-Shaker stained PD nerve, which contains only the axons from the two PD neurons, approaching the PD muscle. The section is ∼6 to 8-μm-thick.