Shal and Shaker channels are targeted to the PD NMJ. A single optical section from an innervated PD muscle double-labeled with a mouse monoclonal antibody against acetylcholinesterase (antiACh; A) and our polyclonal anti-shaker (B). Secondary antibodies were tagged with Oregon Green (anti-mouse) or Texas Red (anti-rabbit). Both fluorescent tags were concurrently imaged in all optical slices of a z-series through the NMJ. Optical section from a second PD muscle double-labeled with anti-AcH(C) and anti-shal(D). Imaging was as described for the previouspanels. Optical sections are ∼0.5 to 2-μm-thick.