Subcellular localization of seladin-1 in H4 human neuroglioma cells. A, D, Subcellular distribution of the green fluorescent seladin-1-EGFP fusion protein.B, Staining of the endoplasmic reticulum with the anti-PDImAb and the red fluorescent CY3-labeled secondary antibody. C, Overlay fromA and B shows the colocalization of seladin-1 with the ER marker, indicated by yellowfluorescence. E, Staining of the mitochondria with thered fluorescence MitoTracker CM-H₂Xros.F, Overlay of D andE.