Expression of traf6 during embryonic development. Northern blot analysis of traf6mRNA prepared from wild-type adult tissues and whole embryos at E7, E11, E15, and E17, showing relative levels of traf6expression. panc, Pancreas; thyr, thyroid; thym, thymus; submx, submaxillary; test, testes; ovar, ovary;prost, prostate; epid, epididymus.B, In situ hybridization of a whole mount of an E9.5 wild-type embryo showing traf6 expression in the developing brain. The highest levels of expression occur in the forebrain (arrowhead), with lower expression in the midbrain and hindbrain regions. C, Rostral view (magnified) of the embryo in B showingtraf6 expression in the developing forebrain (arrowhead).