Downregulated NADE attenuates zinc neurotoxicity.A, Left, Bars represent LDH release in cortical cultures after 20 hr exposure to 25 μm zinc alone (Zinc) or zinc in the presence of 1 μg/ml cycloheximide (+CHX). Asteriskdenotes difference from Zinc (p < 0.05; two-tailed t test). Right, Western blots for p75NTR and NADE in control (CTRL) or after 12 hr exposure to 25 μm zinc without (Zinc) or with 1 μg/ml cycloheximide (+CHX). Western blots for NeuN are presented as control. B, Left, Bars represent LDH release in cortical cultures by additional 20 hr exposure to zinc 25 μm without (Zinc) or with 10 μm of NADE antisense (AS#1+or AS#2+) or 10 μm nonsense (NS+) oligonucleotides. Oligonucleotides were transfected into cortical cells using DOTAP liposomal reagents 3 hr before zinc exposure. Asterisks denote difference from Zinc (p < 0.05; two-tailed ttest with Bonferroni correction for three comparisons).Right, Western blots for NADE in cortical cultures treated with 10 μm of each antisense or nonsense oligonucleotides.
