Correlation between zinc accumulation and p75^NTR/NADE induction. Higher power photomicrographs of the hippocampal CA1 region, sham-operated (top row), 48 hr (middle row), and 72 hr (bottom row) after 15 min ischemia, stained with TFL-Zn (A, D, G), anti-p75^NTRantibody (B, E, H), and anti-NADE antibody (C, F, I). It appeared that zinc accumulation reached its maximum at 48 hr, whereas p75^NTR and NADE immunoreactivity increased between 48 and 72 hr. The NADE immunoreactivity first appeared in the cytoplasm (F), then spread throughout the cell including nuclei (I). In contrast, none of zinc accumulation (A), p75^NTRimmunoreactivity (B), or NADE immunoreactivity (C) was detected in a sham-operated animal. Scale bar, 100 μm.