Dominant-negative ezrin-induced inhibition of migration and invasion: association with altered BCL-2/BAX rheostat, decreased MMP activity, and decreased α_vβ₃integrin expression. A, The levels of BCL-2, BCL-X_L, and BAX in LN-229 whole-cell lysates and of MMP-2, MMP-9, MT1-MMP, and TIMP-2 in the supernatant were examined by immunoblot. MMP-2 activity in conditioned medium of neo, nter-ezrin-, and F353-ezrin-transfected LN-229 cells was examined by gelatin zymography. All blots are representative of experiments performed three times with similar results. B,α_vβ₃ and α₅β₁integrin expression were determined by flow cytometry. The curves for α_Vβ₃ antibody (bold line) and control antibody (dotted line) are shown. As documented in the bottom panel, there was no change in α₅β₁ integrin. In the bar graph, data are expressed as mean SFI values (n = 3).