Inhibition of glioma cell clonogenicity, migration, and invasion by dominant-negative ezrin involves loss of TGF-β₂. A, TGF-β₂ and TGF-β₁ levels in the conditioned medium of neo (lanes 1 and 4) or F353-ezrin or nter-ezrin-transfected LN-229 cells were assessed by immunoblot. In thebottom panels, the levels ofTGF-β₁ andTGF-β₂ mRNA were assessed by semiquantitative RT-PCR. B, C, The migration of LN-229 sublines was measured in the absence (−) or presence (+) of neutralizing TGF-β₂ antibody (2 μg/ml) (B) or the absence (−) or presence (+) of TGF-β₂ (2 ng/ml) (C) (mean and SEM;n = 3; *p < 0.05, **p < 0.01 for the effect of TGF-β₂antibody in B or of TGF-β₂ inC).