Increased TGF-β₂ release by BCL-2-transfected glioma cells. A, The levels of BCL-2 and BAX were measured in whole-cell lysates of LN-229 neo and nter-ezrin cells after no treatment (−) or exposure to TGF-β₂ (2 ng/ml) for 24 hr (+) by immunoblot.B, Released TGF-β₂ was detected in the conditioned medium of LN-229 neo or BCL-2 cells by immunoblot analysis as in Figure 4A.