Expression of the N-terminal domain of TI-VAMP affects the distribution of EAAC1 but not GluR1 in hippocampal neurons.A, Four-day-old hippocampal neurons from E18 rats were transfected with GFP or GFP-Nter-TI-VAMP, and after 24 hr they were fixed and stained for the indicated proteins. Note the expected dendritic localization of EAAC1 in control neurons and in neurons transfected with GFP (right top panel and nontransfected cell in the right middle panel) compared with its general lower expression and specifically its absence from the dendrites in cells expressing Nter-TI-VAMP (transfected cell in theright middle panel). By contrast, the level of expression and the localization of GluR1 were not affected by expression of GFP-Nter-TI-VAMP (compare the two cells in theright bottom panel). Scale bar, 21 μm.B, Cells transfected as in A were stained 24 hr later for EAAC1; shown are the mean values (±SEM) of percentage of GFP- or Nter-TI-VAMP-positive dendrites labeled also for EAAC1.