Effects of ischemia and MnTE-2-PyP⁵⁺ on aconitase–fumarase activities. Rats were subjected to 90 min of middle cerebral artery occlusion and treated with intracerebroventricular vehicle or 300 ng of MnTE-2-PyP⁵⁺ at 5 min or 6 hr after onset of reperfusion. Brains were sampled at 4 hr after treatment, respectively (n = 6–8 per condition). At 4 hr after ischemia, aconitase was selectively decreased by ischemia in the vehicle-treated group. Aconitase activity was 47% greater in animals treated with MnTE-2-PyP⁵⁺. There was no effect of ischemia or MnTE-2-PyP⁵⁺ on fumarase activity. At 10 hr after ischemia, reductions in both aconitase and fumarase activities were observed. Both aconitase and fumarase activities were greater (32 and 16%, respectively) in MnTE-2-PyP⁵⁺ rats versus respective vehicle-treated controls. a, Difference from nonischemic hemisphere (p < 0.05); b, difference from ischemic hemisphere in vehicle-treated group (p < 0.05). Values represent mean ± SD.