Mice were subjected to 90 min of middle cerebral artery occlusion. Five minutes after onset of reperfusion, 0 mg/kg (n = 15), 1 mg/kg (n = 17), or 2 mg/kg (n = 18) MnTE-2-PyP⁵⁺(in PBS vehicle, total injectate volume = 15 μl) was injected intravenously. Infarct volumes for individual mice (open circles) measured at 24 hr after ischemia are shown.Horizontal bars indicate group mean values.Top, Cortical infarct volume was reduced by MnTE-2-PyP⁵⁺ (p = 0.015). An intergroup difference was present for MnTE-2-PyP⁵⁺ (2 mg/kg) versus vehicle (p = 0.017). Middle, Subcortical infarct volume was also reduced by MnTE-2-PyP⁵⁺ (p = 0.013). Intergroup differences were present for both doses versus vehicle (p < 0.04). Total infarct volume was reduced by MnTE-2-PyP⁵⁺(p = 0.009). An intergroup difference was present for MnTE-2-PyP⁵⁺ (2 mg/kg) versus vehicle (p = 0.006). Bottom, Neurologic scores for individual mice are depicted (0, normal; 4, cannot walk spontaneously). Horizontal bars indicate group median values. A main effect for treatment group was present (p = 0.04).