Differential expression of EAG and Kcnq K⁺ channels in brain. NR-ISH for EAG family and Kcnq K⁺ channel transcripts in rat brain using DIG-labeled RNA antisense probes is shown. DIG-labeled probes were detected using the alkaline phosphatase substrate NBT/BCIP for 14 hr. AccN, Accumbens nucleus; CPu, caudate/putamen; Cx, cerebral cortex;Cer, cerebellum; Hipp, hippocampus;IC, inferior colliculus; IO, inferior olive; ob, olfactory bulb; Pn, pontine nucleus; Rt, reticular thalamic nucleus;Th, thalamus; Tu, olfactory tubercle.