Eag1 and eag2 transcripts have overlapping expression in the cerebral cortex, olfactory bulb, and amygdala.A–D, ISH with DIG-labeled eag1 antisense probe.A, Eag1 expression in coronal section at the level of the hippocampus showing strong labeling in the cerebral cortex (Cx), hippocampus (Hipp), and lateral nuclei of the amygdala (La). B, High magnification of A showing eag1 expression in the cerebral cortex with specific lamina identified. Note labeling in layers II–VI, with particular high expression levels in layer IV.C, Eag1 expression in the hippocampus showing strongest signals in the CA2 and CA3 fields and in the dentate gyrus (DG). D, Eag1 staining of the granule cell layer (Gr) of the cerebellum. E–H, ISH using eag2 DIG-labeled antisense probe. E, Eag2 expression in serial section of the same brain as A. Note strong expression in cerebral cortex (Cx) and much weaker signals in the thalamus (Th) and lateral amygdala (La). F, High magnification of the cortex shows eag2 expression in layer IV. Unlike eag1, little or no eag2 expression was found in the hippocampus (G) or the cerebellar cortex (H).I–J, Overlapping expression of eag1 (I) and eag2 (J) transcripts was also found in the internal granule layer (IGr) and the mitral cell layer (Mi) of the olfactory bulb. Scale bar (shown in J):A, E, 1500 μm; B,F, 150 μm; I,J, 500 μm; C, D,G, H, 600 μm.ML, Molecular layer of the cerebellum;Gl, periglomerular layer of the olfactory bulb.