Distribution of mRNA for EAG and Kcnq K+channel subunits in rat brain
| eag1 | eag2 | erg1 | erg2 | erg3 | elk2 | elk3 | Kcnq2 | Kcnq3 | |
|---|---|---|---|---|---|---|---|---|---|
| Forebrain | |||||||||
| Olfactory bulb | |||||||||
| Granular cell layer | ++ | +++ | ++ | − | ++ | ++ | − | ++ | − |
| Mitral cell layer | +++ | +++ | ++ | ++ | ++ | ++ | − | +++ | + |
| Periglomerular layer | − | − | ++ | ++ | ++ | − | − | + | + |
| Piriform cortex | +++ | +++ | + | − | ++ | ++ | ++ | +++ | +++ |
| Anterior olfactory nucleus | +++ | ++ | − | − | + | + | − | +++ | ++ |
| Olfactory tubercle | +++ | + | − | − | ++ | + | ++ | +++ | +++ |
| Cerebral cortex | |||||||||
| Layer II | +++ | + | + | − | +++ | ++ | ++ | +++ | ++ |
| Layer III | +++ | ++ | + | − | +++ | ++ | ++ | +++ | +++ |
| Layer IV | +++ | +++ | + | − | + | +/− | + | +/− | +++ |
| Layer V | ++ | +f | + | − | ++ | + | + | +++ | ++ |
| Layer VI | ++ | − | +/− | − | + | + | + | ++ | + |
| Inhibitory interneurons | − | − | ++1-a | − | − | − | − | +f | − |
| Hippocampus | |||||||||
| Pyramidal cells | |||||||||
| CA1 | ++ | − | ++ | − | +++ | ++ | + | +++ | +++ |
| CA2 | +++ | − | − | − | − | +/− | − | +++ | + |
| CA3 | +++ | +/− | − | − | − | +/− | − | +++ | +++ |
| DG | ++ | +/− | − | − | − | ++ | + | +++ | +++ |
| Inhibitory interneurons | − | − | ++ | − | − | − | − | − | − |
| Septum | + | +/− | ++ | − | +/− | − | − | ++ | + |
| Basal ganglia | |||||||||
| Caudate/putamen | ++ | − | Int | − | +/− | + | ++ | ++ | +++ |
| Accumbens nucleus | ++ | − | − | − | − | − | ++ | ++ | ++ |
| Globus pallidus | − | − | + | − | − | − | − | +/− | +/− |
| Thalamus | |||||||||
| Medial geniculate nucleus | − | ++ | ++ | − | ++1-b | − | − | ++ | +++ |
| Ventral posterior thalamic complex | − | ++ | ++ | − | +/− | − | − | ++ | +++ |
| DLG | − | ++ | ++ | − | +/− | − | − | ++ | +++ |
| Reticular thalamic nucleus | − | − | +++ | − | ++ | − | − | ++ | +++ |
| Habenula | + | ++ | ++ | − | + | − | − | ++ | − |
| Mammilary nucleus | +++ | − | ++ | − | − | − | − | + | + |
| Amygdala | ++ | ++1-c | + | − | + | ++ | − | ++ | ++ |
| Hypothalamus | ++ | + | ++ | − | +/− | − | − | ++ | ++ |
| Midbrain | |||||||||
| Superior colliculus | |||||||||
| SuG | − | + | + | − | ++ | + | − | + | ++ |
| InG | − | ++ | + | − | ++ | + | − | + | ++ |
| Inferior colliculus | + | +++ | +++ | − | + | − | − | ++ | ++ |
| Substantia nigra | |||||||||
| Pars reticulata | − | − | +f | − | − | − | − | +f | ++ |
| Pars compacta | − | − | ++ | − | − | − | − | ++ | + |
| Nucleus lateral lemniscus | − | +++ | +++ | − | +++ | − | − | + | + |
| Ventral tegmental nucleus | − | − | +++ | − | − | − | − | +++ | + |
| Raphe nucleus | − | + | +++ | − | ++ | − | − | + | ++ |
| Central gray | + | + | + | − | + | − | − | + | + |
| Red nucleus | ++ | − | +++ | − | − | − | − | ++ | +++ |
| Oculomotor nucleus (3) | ++ | − | +++ | − | − | − | − | ++ | + |
| Hindbrain | |||||||||
| Cerebellum | |||||||||
| Granule layer | +++ | − | ++ | − | − | ++ | − | +++ | − |
| Molecular layer | − | − | − | − | − | − | − | − | − |
| Purkinje cell layer | − | − | +++ | − | ++ | − | − | +++ | − |
| Deep nuclei | + | − | +++ | − | + | − | − | ++ | +/− |
| Pontine nucleus | + | ++ | +++ | − | − | − | − | ++ | + |
| Superior olive | ++ | ++ | ++ | − | + | − | − | + | − |
| Vestibular nucleus | + | +/− | +++ | − | − | − | − | + | + |
| Dorsal cochlear nucleus | − | ++ | +++ | − | − | − | − | ++ | ++ |
| Ventral cochlear nucleus | − | ++ | +++ | − | − | − | − | +/− | + |
| Facial nucleus (7) | ++ | + | +++ | − | − | − | − | ++ | ++ |
| Spinal trigeminal nucleus (5) | − | + | +++ | − | + | − | − | ++ | + |
| Gracile nucleus | − | − | +++ | − | ++ | − | − | + | + |
| Cuneate nucleus | + | + | +++ | − | ++ | − | − | ++ | + |
| Dorsal motor nucleus, vagus (10) | − | − | + | − | ++ | − | − | − | − |
| Gigantocellular reticular nucleus | +/− | +/− | +++ | − | +/− | − | − | + | + |
| Hypoglossal nucleus (12) | + | − | ++ | − | − | − | − | ++ | + |
| Lateral reticular nucleus | − | + | +++ | − | ++ | − | − | ++ | + |
| Inferior olive | − | − | +++ | − | ++ | − | − | ++ | ++ |
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The data for this table (except for Kcnq2) were generated from NR-ISH of serial coronal sections from the same rat brain in which all genes were processed in parallel in the same experiment under identical conditions. Relative levels of expression were first determined for each individual gene and then normalized between the different genes. +++, Very high; ++, moderate to high; +, low, +/−, just detectable; −, not detectable; f, few neurons; Int, in PV+ interneurons.
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↵F1-a Mainly in cingulate cortex.
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↵F1-b In amygdala projection nucleus of medial geniculate only.
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↵F1-c Eag2 was particularly strong in the intercalated nucleus.
