Transient expression of α2-chimaerin, its SH2 and GAP domain mutants, and α1-chimaerin in N1E-115 neuroblastoma cells. Cells plated on poly-lysine were transfected with pXJ40-GFP-α2-chimaerin, SH2 domain mutants E49W, R56L, R73L, N94H, α1-chimaerin, α2-chimaerin GAP domain mutant α2R304G, or α2N94HR304G SH2/GAP double mutant (or GFP; data not shown). Transfectants were cultured in DMEM and 5% FCS for 24 hr, stained with phalloidin, and analyzed by confocal microscopy. GFP fluorescence and corresponding F-actin are shown.