Effects of Rac1 N17 and Cdc42 N17 (and chimaerin N94H) on chimaerin cell lines. A, Permanent α2-chimaerin (b–d) and α1-chimaerin cell lines (g–j) were transiently transfected with GFP-Rac1 N17 (b, g, i) or HA-Cdc42 N17 (c, h, j) and stained with phalloidin and (where appropriate) anti-HA antibody. Cells were plated on poly-lysine (b–h) or laminin (i, j). Permanent α2-chimaerin cell lines transiently transfected with GFP-chimaerin N94H were phalloidin-stained (d). Morphology of permanent α2-chimaerin cells plated on poly-lysine (a) and permanent α1-chimaerin-expressing cell lines plated on poly-lysine (e) or laminin (f) and transiently transfected with GFP-tagged constructs, as indicated, were quantitated in five morphology categories: round (white columns), microspikes (black columns), flattened (light gray columns), ruffled (dark gray columns), and with neurites >2 cell diameter (striped columns) as a percentage of (GFP-expressing) transfected cells.