A, Odorant responses of the mOR-EG. Various odorants (1 mm) were applied for 20 sec at the time indicated by arrows. The mOR-EG shows responses to EG, vanillin, and EV in HEK293T cells cotransfected with Gα15/16, as shown by changes in fura-2 fluorescence intensity ratios. Isoproterenol (Iso) (10 μm) serves as a control for Gα15/16 cotransfection. 1, Isoeugenol;2, guaiacol; 3, safrol; 4, allylbenzene; 5, syringic aldehyde; 6, vanillic acid. The structures of these compounds were drawn in Figure5. B, Dose-dependent Ca2+ responses of the mOR-EG to various ligand odorants. The responses of mOR-EG to increased concentrations of EG, vanillin, and EV in HEK293T cells cotransfected with Gα15/16, as shown by changes in fura-2 fluorescence intensity ratios.
