Pharmacological characterization of the S-IPSCs and of the L-IPSCs recorded from Golgi cells.A, Gabazine blocks spontaneous IPSCs but not serotonin-evoked IPSCs. The top trace represents 5 min of recordings during which gabazine (30 μm) and serotonin (1 μm) were bath applied at the time indicated byhorizontal bars. The asterisk and thefilled circle indicate the time at which thebottom traces, displayed at a faster time scale, were taken. CNQX (3 μm) was present during the whole experiment to block all EPSCs. B, Summary of a similar experiment in another cell illustrates that strychnine blocks L-IPSCs induced in the presence of gabazine. The cumulative amplitude represents the sum of the peak amplitudes of the IPSCs.C, GABAergic L-IPSCs can be evoked in the presence of strychnine. The top trace represents 4 min of continuous recording in the presence of strychnine and during application of serotonin (1 μm). Bottom traces display the inhibitory activity, at a faster time scale, at the times indicated by the asterisk and filled circle. In this and the following figures, all of the cells were recorded in the whole-cell configuration of the patch-clamp technique with a CsCl-based internal solution and were held at −70 mV.