Glycine receptor immunoreactivity of the cerebellar Golgi cell. A, Low-magnification micrograph of a cerebellar section cut in the sagittal plane and immunoreacted with the mAb 4a. Golgi cell somata (arrows) in the granular layer and dendrites (arrowheads) in both granular and molecular layers appear stained for GlyRα/β, as well as some glomerular structures of the granular layer (crossed arrows). B, High magnification of a Golgi cell (reconstruction) located in the upper granule cell layer, showing diffuse staining of the cell body (arrow) and punctate labeling over the dendritic tree in the molecular layer (arrowheads). Note that only Golgi cell dendrites are stained in the molecular layer. The asterisk indicates a Golgi cell body out of focus. C, Diffuse intracellular staining of the Golgi cell body and initial dendritic portion, in contrast with the intense, punctiform labeling of dendrites (arrowheads). Note that the nucleus (n) is unstained. D, Labeled dendrites of Golgi cells in the molecular layer (arrowheads). E, Double immunodetection of GlyRα/β (E1) and VIAAT (E2) in the molecular layer. The slight shift in the immunoreactivity of the two markers indicates their close apposition (arrowheadsshow the location of VIAAT-positive presynaptic terminals). VIAAT-labeled terminals (arrows) not associated with glycine receptor clusters are thought to be involved in the numerous GABAergic synapses in the molecular layer. g, Granular cell layer; m, molecular layer; P, Purkinje cell layer; wm, white matter. Scale bars:A, 50 μm; B, 25 μm; C, 10 μm; D, 20 μm; E, 5 μm.