A, B, Colocalization of GlyR and gephyrin with GABA_AR clusters at Golgi cell dendrites in the low molecular layer is shown. Sagittal sections were double-stained with anti-GABA_ARγ2 antibody (greenin A1; red in B1) and anti-GlyRα/β (red in A2) or anti-gephyrin (green in B2) antibodies. A3 and B3 are the superimposed images of A1 and A2 and ofB1 and B2, respectively.A, Clusters positive for the two receptors are intermingled at single postsynaptic sites (A3,arrowheads). B, Clusters of GABA_ARγ2 are closely associated to, but do not colocalize with, gephyrin aggregates at putative postsynaptic sites on Golgi cell dendrites (arrowheads). In the insets(solid rectangles) of A3 andB3, a 2.5× magnification of a region where the labeling is representative (dashed rectangle) is shown enlarged.C–E, Lugaro to Golgi cell axonal contacts are shown. Transverse (C, D) or sagittal (E) sections were double-stained with anti-calretinin (green in C1) or anti-GlyT2 (green in D1, E1) antibodies and with an anti-GlyRα/β antibody (red inC2, D2, E2).C3, D3, and E3 are the superimposed images of C1 and C2,D1 and D2, and E1 andE2, respectively. C, D, Axonal calretinin-stained (C) and GlyT2-stained (D) varicosities are found apposed to GlyRα/β-immunoreactive clusters (arrowheads). Theinsets in C, taken from another section, show a contact at a higher magnification (5×). Note that a few terminals are not associated with GlyR (arrows).E, A GlyT2-immunoreactive varicose fiber from the Lugaro cell sagittal axonal plexus is shown. Some GlyT2 terminals are apposed to GlyR clusters (arrowheads), whereas others are not (arrows). The latter probably correspond to contacts between the Lugaro cell sagittal axon and molecular layer interneurons.F, Double immunostaining of parvalbumin (F1) and glycine receptor (F2) in the molecular layer is shown. Clusters of glycine receptor (arrows) are not associated with parvalbumin-immunoreactive profiles, belonging either to basket, stellate, or Purkinje cells (double arrowheads).Asterisks indicate the cell bodies of the molecular layer interneurons. Pva, Parvalbumin. Scale bars: A, B, F, 5 μm; C–E (bar inF), 10 μm.