A, Schematic diagram of isolated lobster stomatogastric nervous system used in long-term organotypic culture. The stn was cut (arrow) in decentralized preparations and left intact in control preparations.CoG, Commissural ganglia; OG, esophageal ganglion; STG, stomatogastric ganglion; stn, stomatogastric nerve; lvn, lateral ventricular nerve;pdn, pyloric dilator nerve; vlvn, ventral lateral ventricular nerve. B, Pyloric network activity recorded from indicated nerves on day 1 in vitro before (B1) and after (B2) thestn was cut, and from the same preparation on day 6 in organ culture (B3). (Note spikes from the tonically active anterior gastric receptor in all stnrecordings.)