Identified PD neurons express shal-like immunoreactivity after STG decentralization. A, Double fluorescent staining of a single PD neuron injected with dextran tetramethylrhodamine (A1, A2,A4) and treated with Cy5-labeled anti-shal antibody (A3, A5) in a control preparation. Images are maximal projections composed of six optical sections representing a total depth of 12 μm. Image stacks inA2 and A3 were acquired at the level of neurites in the STG (A1, square), whereasA4 and A5 were taken at the level of STG somata. No shal staining was evident on either the PD cell body or its neurites. * indicates unidentified neurite that showed shal staining.B, Similar double staining to that shown inA, but from a decentralized preparation. Shal staining was present on both the somata (B5) and large neurites (B3) of the rhodamine-injected PD neuron.A1 and B1 are at same scale, as areA2–5 andB2–5. C, Percentage of identified PD neuron somata expressing shal-like immunoreactivity was higher in long-term decentralized STG relative to control preparations.